| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bruzgul, Jaxon R (12)
|
NITWST |
26.81 |
26.61 |
-0.20 |
| 2 |
Bembenista, Owen A (12)
|
NITWST |
29.89 |
29.01 |
-0.88 |
| 3 |
Kirchberger, Luke A (12)
|
NIHAMB |
31.44 |
30.14 |
-1.30 |
| 4 |
Mallory, John B (12)
|
NITWST |
29.38 |
30.64 |
1.26 |
| 5 |
Carpenter, Trenten E (12)
|
NIRAYS |
|
32.29 |
- |
| 6 |
Gatti, Jake D (12)
|
NIUN |
33.88 |
33.25 |
-0.63 |
| 7 |
Metro, Ethan M (11)
|
NIEAST |
35.08 |
32.90 |
-2.18 |
| 8 |
Weseman, Gavin L (12)
|
NIEMAC |
33.70 |
33.04 |
-0.66 |
| 9 |
Barrett, David L (11)
|
NIHHSC |
31.91 |
33.45 |
1.54 |
| 10 |
Breton, Dominic C (12)
|
NIFKVL |
35.50 |
34.75 |
-0.75 |
| 11 |
Merrill, Christian T (12)
|
NIGWST |
34.45 |
35.35 |
0.90 |
| 12 |
Andrew, Jacob D (12)
|
NIHAMB |
34.16 |
35.45 |
1.29 |
| 13 |
Bubar, Isaac J (11)
|
NITWST |
36.88 |
35.57 |
-1.31 |
| 14 |
Boyle, William R (11)
|
NITTSC |
36.01 |
36.28 |
0.27 |
| 15 |
Suffoletto, Adam P (11)
|
NITWST |
38.08 |
36.82 |
-1.26 |
| 16 |
Bloom, Anthony D (11)
|
NITWST |
37.02 |
36.97 |
-0.05 |
| 17 |
Sippel, Patrick N (11)
|
NITWST |
38.85 |
38.32 |
-0.53 |
| 18 |
Endress, Elton P (12)
|
NIHAMB |
37.63 |
38.41 |
0.78 |
| 19 |
Cleveland, Landon K (11)
|
NIPVSC |
35.27 |
39.63 |
4.36 |
| 20 |
Ramsey, Tamias E (12)
|
NITTSC |
41.58 |
40.44 |
-1.14 |
| 21 |
Tareen, Andrew J (11)
|
NITTSC |
|
46.10 |
- |
| 22 |
Askar, Joe I (11)
|
NIHHSC |
45.70 |
52.41 |
6.71 |